High Performance liquid Chromatography also known as HPLC is a widely used instrument for analysis in pharmaceutical, chemical and food industries. It has powerful ability to identify and quantify the complex mixtures and this makes it an essential instrument for analytical laboratories. This article will provide detailed knowledge on major components, working and principle of HPLC . What is HPLC ? HPLC system is an advanced version of column chromatography that is used to separate, identify ... What does HPLC mean? High-Performance Liquid Chromatography ( HPLC ) or High-Pressure Liquid Chromatography, is a technique in analytic chemistry used to separate the components in a mixture, to identify each component, and to quantify each component. Introduction High-performance liquid chromatography ( HPLC ) is an indispensable analytical technique in the pharmaceutical industry. It allows for the separation, identification, and quantification of the chemical components of pharmaceutical drug products and drug substances. With its high sensitivity, accuracy, and reproducibility, HPLC enables pharmaceutical companies to analyze everything from drug raw materials, intermediates, and final products to perform quality control, optimize ... What is HPLC (High-performance liquid chromatography)? High-Performance Liquid Chromatography ( HPLC ) is a sophisticated analytical technique used extensively in various scientific fields to separate, identify, and quantify the components of a mixture. This technique, originally known as high-pressure liquid chromatography, has evolved significantly since its inception. HPLC operates on the principle of column chromatography but utilizes advanced technology to enhance its performance. Unlike ...