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A hemocytometer, also known as a hemacytometer or haemocytometer, is a glass microscope slide used for manually counting cells in a cell culture sample. To help researchers count the cells methodically and accurately, the hemocytometer contains a grid of varying sized squares and perpendicular lines. Many biological applications that use cells, such as microbiology, cell culture, and blood work, require that we determine cell concentration for our experiments. Cell counting is actually quite straightforward and requires a counting chamber called a hemocytometer, a device invented by the 19th-century French anatomist Louis-Charles Malassez to perform blood cell counts. A hemocytometer consists of a thick glass microscope slide with a grid of perpendicular lines etched in the middle. The ... Learn how to use a hemocytometer, a specialized slide for counting cells, in microbiology, biotechnology, and pathology. Find out the design, structure, and rules of a hemocytometer and how to calculate the number of cells in a sample. Learn how to use cell counting hemocytometer with our step-by-step guide. Hemocytometer calculation determines cell concentration, viability, and thawing results.