Tissue culture is defined as a technique used to grow cells, tissues, or organs in a controlled, sterile environment. It allows scientists to produce thousands of identical plants from a single parent plant, making it a powerful tool in plant propagation. This method is especially useful in agriculture as part of strategies for enhancement in food production, helping in the rapid multiplication of high-yielding and disease-resistant crops. Tissue culture produces clones, in which all product cells have the same genotype (unless affected by mutation during culture ) An Introduction to Plant Tissue Culture : Advances and Perspectives What is plant tissue culture and why it matters Plant tissue culture refers to the aseptic cultivation of plant cells, tissues, or organs on defined nutrient media under controlled environmental conditions. In tissue culture , cells or tissues are removed from an organism and grown in the laboratory using a nutrient medium that provides the nutrients and growth factors the cells need to grow.